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M94A3298.TXT
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1994-10-25
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Document 3298
DOCN M94A3298
TI Proviral DNA in virions from primary isolates.
DT 9412
AU Asjo B; Sommerfelt M; National Virus Center, University of Bergen,
Norway.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):108 (abstract no. PA0052). Unique
Identifier : AIDSLINE ICA10/94369277
AB OBJECTIVE: To determine whether proviral DNA is present in HIV virions
of primary isolates grown in primary cells. MATERIALS AND METHODS:
Patient isolates with slow/low and rapid/high type characteristics were
used to infect PHA blasts. Virions were collected by ultracentrifugation
and subjected to three different treatments prior to PCR amplification
using primers hybridizing with U5 and R regions in the LTR (recognizing
strong-stop DNA) and the pol region. Negative control consisted of
pelleted disrupted virions treated with DNAse to remove adherent
contaminating proviral DNA from disrupted cells. RESULTS: Proviral DNA
corresponding to strong-stop DNA was demonstrated in virions from
primary isolates of both slow/low and rapid/high phenotype. No signal
could be detected with primers to the pol region which is in agreement
with previous data that only partial reverse transcription takes place
within the virion. CONCLUSION: Our data show that strong-stop DNA
synthesis is not only a phenomenon associated with laboratory adapted
strains of HIV but is also present in primary isolates grown in primary
cells. It most likely represents a phenomenon common to the retrovirus
family.
DE Cells, Cultured Deoxyribonucleases DNA,
Viral/BIOSYNTHESIS/GENETICS/*ISOLATION & PURIF Genes, pol Human
HIV/GENETICS/*ISOLATION & PURIF HIV Long Terminal Repeat Phenotype
Polymerase Chain Reaction Proviruses/GROWTH &
DEVELOPMENT/GENETICS/*ISOLATION & PURIF Virus Cultivation MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).